Consumables

Vials and Septa

A known fact in the glass industry is that not all glass is the same. Besides the compounds contained in the glass, the manufacturing process can widely change the properties of the final product. Sodium is a common element found in glass and is the major cause of surface adsorption on the glass. All adsorption in borosilicate glass is polar in nature causing sticking and bonding to the glass surfaces. Highly negatively charged hydroxyl sites in the glass matrix yield the perfect binding sites for positively charged compounds to stick to the glass surfaces.

Two manufacturing steps need to occur to produce a Low Adsorption (LA) vial that can be “Mass Spec Certified”. Step one is to remove as much of the metals as possible with particular attention to sodium. The sodium as well as other metals can leach out of the glass into the liquid in the vial and chemically bond with certain common solvents. When this leaching occurs, the pH of the solution increases and can cause degradation to pH sensitive analytes. Vials have been shown to contain 28 times less sodium than competitor vials which correlates to competitor vials having typical pH increases of up to 2.8 units or more. The second step in the process is to silanize or coat the glass in order to increase hydrophobicity.

Modern septa are manufactured using a double layer design with the top layer for sealing and the bottom layer for sample protection.

    Two Types of Septa

  • The first and most common septum is one consisting of a silicone top layer and a PTFE bottom layer.
  • The second septum is a silicone top layer with a polypropylene bottom layer.

    The main goals when choosing a vial or microtiter plate.
  • Hold the sample without allowing it to adsorb into the container.
  • Reduce the effects of extractables.
  • Prevent leaching of materials from the container into the sample.

Features of Vials and Septa
  • 1st hydrolytic class and silanized glass.
  • Vials are packaged in a cleanroom.
  • Contamination-free septa production.

Fittings for HPLC Column

With an innovative design utilizing stainless steel or a proprietary PEEK™ polymer blend (PK), Fittings ensure outstanding performance for both conventional and demanding analyses. These reusable fittings, including the new Nexlock Finger-Tight fittings, range in pressure ratings from 5,000 to 25,000 psi, allowing analysts to select the best option for their application needs.

Column End Plugs

These Column End Plugs fit all columns with 10-32 threads. The long design and threading down to the ferrule ensure safe closing of valuable columns. Seal your unused columns against contamination or evaporation. These sturdy fingertight plugs are made to hold tight, even if you drop your column!

  • Available with a 5/16" wrench flat.
  • Easy removal.
  • Red, Green, Yellow, Black & Blue coloured column fittings.

Tubing

Tubing is an excellent alternative to stainless steel and is usable in multiple applications. Key advantages include flexibility, bio-compatibility, high temperature and chemical resistance, and is easily cut to desired lengths.