ACME HPLC/UHPLC columns are based on a new 120 Å ultra-high purity (99.999%) extra-treated porous spherical silica with a narrow particle size distribution and an extremely low metal content(< 10 ppm) to minimize silanol acidity and reduce surface metal sites available for chelation. ACME PLUS HPLC/UHPLC are based on the same ultra-high purity silica with a pore size of 200 Å.
Combined with a proprietary bonding technology this results in columns with optimal efficiency, asymmetry, and minimal back pressure. ACME silica pore structure shows dramatically improved performance on real samples.
C18* | F5/C18 | Amide/C18 | |
---|---|---|---|
Bonded Phase | -C18H37 | -C18H37 –X-PFP |
–(CH2)3NH(C=O)-C15H37 |
Separation Mode | Reversed-Phase | ||
Base Material | Ultrapure Extra-Treated Porous Spherical Silica | ||
Purity (%) | 99.999, metals < 10 ppm | ||
Surface Area | 260 m2/g | ||
Particle Size (s) | 1.9 µm, 3 µm, 5 µm | ||
Pore Size | 120 Å | ||
Carbon Load (%) | 16 | 16 | 19 |
Endcapping | Yes Proprietary | ||
pH Range | 1.8 to 9.5 | ||
Temperature Limit | 60 °C | ||
Aqueous Compatibility | ≤ 98% | 100% | 100% |
USP Listing | L1 | L1/L43 | L1/L60 |
ACME C18 | ACME C8 | ACME CN* | ACME Ph | ACME PLUS | ACME PAH | |
---|---|---|---|---|---|---|
Bonded Phase | -C18H37 | -C8H17 | -R-CN | -R-C6H5 | -C18H37 | -(C18H37)x |
Separation Mode | Reversed-Phase | |||||
Base Material | Ultrapure Extra-Treated Porous Spherical Silica | |||||
Purity (%) | 99.999, metals < 10 ppm | |||||
Surface Area | 260 m2/g | 200 m2/g | ||||
Particle Size (s) | 1.9 µm, 3 µm, 5 µm | |||||
Pore Size | 120 Å | 200 Å | ||||
Carbon Load (%) | 16 | 10 | 7 | 10 | 12 | 17 |
Endcapping | Yes Proprietary | Yes Proprietary | Yes Proprietary | Yes Proprietary | Yes Proprietary | No |
pH Range | 1.8 to 9.5 | 1.8 to 9.5 | 2 to 7.5 | 1.8 to 9.5 | 1.8 to 9.5 | 1.8 to 9.5 |
Temperature Limit | 60 °C | 60 °C | 50 °C | 60 °C | 60 °C | 60 °C |
Aqueous Compatibility | ≤ 98% | ≤ 98% | 100% | 100% | ≤ 98% | ≤ 98% |
USP Listing | L1 | L7 | L10 | L11 | L1 | L1 |
Application | Phase | Particle Size | Column Length | Column ID |
---|---|---|---|---|
Analgesics/Aspirin Mix(Hydrophilic Acids) | Amide/C18 | 3 | 100 | 4.6 |
Analgesics & Hydrophobic Acid | Amide/C18 | 3 | 100 | 4.6 |
Aqueous Conditions | F5/C18 | 3 | 100 | 4.6 |
Benzodiazepines | C18 | 3 | 100 | 2.1 |
F5/C18 | 3 | 100 | 4.6 | |
Amide/C18 | 3 | 100 | 4.6 | |
Benzoic Acid/Phenylacetic Acid | Amide/C18 | 3 | 100 | 4.6 |
Beta Blockers | C18 | 1.9 | 100 | 2.1 |
Bisphenols | Amide/C18 | 3 | 100 | 2.1 |
Caffeine Metabolites | C8/C18 | 3 | 100 | 4.6 |
Cannabinoids | C18 | 3 | 150 | 4.6 |
PLUS | 3 | 50 | 2.1 | |
Clinical - VMA, HVA & 5-HIAA | F5/C18 | 1.9 | 100 | 2.1 |
Cortisones | C18 | 3 | 50 | 4.6 |
Diazepam Metabolites | C18 | 1.9 | 100 | 2.1 |
Explosives | F5/C18 | 3 | 100 | 4.6 |
Fat Soluble Vitamins | Amide/C18 | 3 | 100 | 4.6 |
Fluoxetine Metabolites | CN | 3 | 100 | 4.6 |
Hydrophobic Bases | Amide/C18 | 3 | 100 | 4.6 |
Hydrophobic Retention | F5/C18 | 3 | 100 | 4.6 |
Methoxy Isomers | F5/C18 | 3 | 100 | 4.6 |
NSAIDS / Diuretics | C18 | 1.9 | 50 | 4.6 |
Nucleosides / Nucleotides | F5/C18, Amide/C18 | 3 | 100 | 4.6 |
Paracetamol / Aspirin Mix | C18 | 1.9 | 50 | 2.1 |
Phenols | C18 | 5 | 150 | 4.6 |
Ph | 3 | 100 | 4.6 | |
F5/C18 | 3 | 100 | 4.6 | |
Amide/C18 | 3 | 100 | 4.6 | |
Phenols & Carboxylic Acids | Amide/C18 | 3 | 100 | 4.6 |
Polycyclic Aromatic Hydrocarbons | PAH | 3 | 100 | 4.6 | QA Test Mix | Amide/C18 | 3 | 150 | 4.6 | F5/C18 | 3 | 150 | 4.6 |
Selectivity Analytes | Ph | 3 | 100 | 4.6 |
THC Metabolites / Isocratic | C8/C18 | 3 | 100 | 4.6 |
Tricyclic Antidepressants | C18/C8/CN/Ph | 3 | 100 | 4.6 |
Vanilla | Amide/C18 | 3 | 100 | 4.6 |
Water Soluble Vitamins | C18 | 3 | 100 | 4.6 |
PLUS | 3 | 100 | 4.6 | |
Xanthines | C18 | 3 | 50 | 4.6 |