ACME

ACME

ACME HPLC/UHPLC columns are based on a new 120 Å ultra-high purity (99.999%) extra-treated porous spherical silica with a narrow particle size distribution and an extremely low metal content(< 10 ppm) to minimize silanol acidity and reduce surface metal sites available for chelation. ACME PLUS HPLC/UHPLC are based on the same ultra-high purity silica with a pore size of 200 Å.

Combined with a proprietary bonding technology this results in columns with optimal efficiency, asymmetry, and minimal back pressure. ACME silica pore structure shows dramatically improved performance on real samples.

Features

  • Ultrapure porous silica particles enable high peak efficiency across all particle sizes.
  • 1.9, 3, 5 µm particle sizes for simple method transfer between HPLC & UHPLC.
  • Column hardware compatible with HPLC and UHPLC.
  • Consistent and reproducible retention times allowing direct scale-up from the laboratory through process applications.

Specifications

C18* F5/C18 Amide/C18
Bonded Phase -C18H37 -C18H37
–X-PFP		 –(CH2)3NH(C=O)-C15H37
Separation Mode Reversed-Phase
Base Material Ultrapure Extra-Treated Porous Spherical Silica
Purity (%) 99.999, metals < 10 ppm
Surface Area 260 m2/g
Particle Size (s) 1.9 µm, 3 µm, 5 µm
Pore Size 120 Å
Carbon Load (%) 16 16 19
Endcapping Yes Proprietary
pH Range 1.8 to 9.5
Temperature Limit 60 °C
Aqueous Compatibility ≤ 98% 100% 100%
USP Listing L1 L1/L43 L1/L60
ACME C18 ACME C8 ACME CN* ACME Ph ACME PLUS ACME PAH
Bonded Phase -C18H37 -C8H17 -R-CN -R-C6H5 -C18H37 -(C18H37)x
Separation Mode Reversed-Phase
Base Material Ultrapure Extra-Treated Porous Spherical Silica
Purity (%) 99.999, metals < 10 ppm
Surface Area 260 m2/g 200 m2/g
Particle Size (s) 1.9 µm, 3 µm, 5 µm
Pore Size 120 Å 200 Å
Carbon Load (%) 16 10 7 10 12 17
Endcapping Yes Proprietary Yes Proprietary Yes Proprietary Yes Proprietary Yes Proprietary No
pH Range 1.8 to 9.5 1.8 to 9.5 2 to 7.5 1.8 to 9.5 1.8 to 9.5 1.8 to 9.5
Temperature Limit 60 °C 60 °C 50 °C 60 °C 60 °C 60 °C
Aqueous Compatibility ≤ 98% ≤ 98% 100% 100% ≤ 98% ≤ 98%
USP Listing L1 L7 L10 L11 L1 L1

Application

Application Phase Particle Size Column Length Column ID
Analgesics/Aspirin Mix(Hydrophilic Acids) Amide/C18 3 100 4.6
Analgesics & Hydrophobic Acid Amide/C18 3 100 4.6
Aqueous Conditions F5/C18 3 100 4.6
Benzodiazepines C18 3 100 2.1
F5/C18 3 100 4.6
Amide/C18 3 100 4.6
Benzoic Acid/Phenylacetic Acid Amide/C18 3 100 4.6
Beta Blockers C18 1.9 100 2.1
Bisphenols Amide/C18 3 100 2.1
Caffeine Metabolites C8/C18 3 100 4.6
Cannabinoids C18 3 150 4.6
PLUS 3 50 2.1
Clinical - VMA, HVA & 5-HIAA F5/C18 1.9 100 2.1
Cortisones C18 3 50 4.6
Diazepam Metabolites C18 1.9 100 2.1
Explosives F5/C18 3 100 4.6
Fat Soluble Vitamins Amide/C18 3 100 4.6
Fluoxetine Metabolites CN 3 100 4.6
Hydrophobic Bases Amide/C18 3 100 4.6
Hydrophobic Retention F5/C18 3 100 4.6
Methoxy Isomers F5/C18 3 100 4.6
NSAIDS / Diuretics C18 1.9 50 4.6
Nucleosides / Nucleotides F5/C18, Amide/C18 3 100 4.6
Paracetamol / Aspirin Mix C18 1.9 50 2.1
Phenols C18 5 150 4.6
Ph 3 100 4.6
F5/C18 3 100 4.6
Amide/C18 3 100 4.6
Phenols & Carboxylic Acids Amide/C18 3 100 4.6
Polycyclic Aromatic Hydrocarbons PAH 3 100 4.6
QA Test Mix Amide/C18 3 150 4.6
F5/C18 3 150 4.6
Selectivity Analytes Ph 3 100 4.6
THC Metabolites / Isocratic C8/C18 3 100 4.6
Tricyclic Antidepressants C18/C8/CN/Ph 3 100 4.6
Vanilla Amide/C18 3 100 4.6
Water Soluble Vitamins C18 3 100 4.6
PLUS 3 100 4.6
Xanthines C18 3 50 4.6